Edwards, Bruce S., PhD

EDUCATION
B.S. (Biology) 1971, Birmingham Southern College
Ph.D. (Biology) 1979, University of Colorado, Boulder


 

EMPLOYMENT

2005 - Present Leader, High Throughput Screening Core, New Mexico Molecular Libraries Screening Center (NIH MLSCN), Albuquerque, NM
2003 - Present Research Professor, Department of Pathology, University of New Mexico School of Medicine, Albuquerque, NM
2003 - Present Director, Core Flow Cytometry Facility, Cancer Research and Treatment Center, Albuquerque, NM
2002 - Present Adjunct Associate Scientist, Lovelace Respiratory Research Institute, Albuquerque, NM
1997 - Present Research Associate Professor, Department of Pathology, University of New Mexico School of Medicine, Albuquerque, NM
1997 - Present Scientific Coordinator, Shared Flow Cytometry Resource, Cancer Research and Treatment Center, Albuquerque, NM
1991 - 1997 Adjunct Associate Professor, University of New Mexico Cancer Research and Treatment Center, Albuquerque, NM
1994 - 1997 Adjunct Associate Professor, Department of Pathology, University of New Mexico School of Medicine, Albuquerque, NM
1989 - 1997 Scientist, Research Division, The Lovelace Institutes, Albuquerque, NM
1984 - 1997 Director, Flow Cytometry Facility, The Lovelace Institutes, Albuquerque, NM
1987 - 1989 Associate Scientist II, Research Division, The Lovelace Institutes, Albuquerque, NM
1984 - 1987 Associate Scientist I, Research Division, The Lovelace Institutes, Albuquerque, NM
1983 Assistant Scientist, Human Oncology, Wisconsin Clinical Cancer Center, Madison, WI
1979 - 1983 Research Associate, Human Oncology, Wisconsin Clinical Cancer Center, Madison, WI
1975 - 1979 Teaching Assistant, Department of EP&O Biology, University of Colorado, Boulder, CO

 

HONORS
NIH Postdoctoral Research Trainee/Fellow, 1979-1981
Cetus/Shell-Triton Postdoctoral Research Fellowship, 1982
Scientific Staff Research Award, The Lovelace Institutes, 1991


 

AREAS OF RESEARCH
General areas of research interest include cellular immunology, signal transduction, and cell adhesion. Research projects include studies of cell adhesion mechanisms by which eosinophils migrate into lungs to promote asthma, investigation of processes governing leukocyte integrin-dependent cell adhesion, and molecular pharmacology of G-protein coupled receptors of the formylpeptide receptor family. Another area of current active interest is the development of hardware and software systems to improve flow cytometry technology. Recent examples include NIH funded development of the Plug Flow and HyperCyt™ Cytometry systems for automated high throughput analysis of cells and bio-particles, the basis of screening technologies currently used for drug discovery in the NIH-sponsored New Mexico Molecular Libraries Screening Center.

 

PATENTS

  1. US Patents #6,315,952 (Nov 13,2001) and #6,638,481 (Oct 28, 2003) - Plug flow cytometry for high throughput screening and drug discovery.

     

  2. US Patents #6,878,556 (Apr 12, 2005) and #6,890,487(May 10, 2005) - Flow cytometry for high throughput screening.

 

REPRESENTATIVE PUBLICATIONS

  1. Edwards, B. S., F. W. Kuckuck, E. R. Prossnitz, A. Okun, J. T. Ransom and L. A. Sklar: Plug Flow Cytometry Extends Analytical Capabilities in Cell Adhesion and Receptor Pharmacology. Cytometry 43: 211-216, 2001.

     

  2. Edwards, B. S., F. W. Kuckuck, E. R. Prossnitz, J. T. Ransom and L. A. Sklar: HTPS Flow Cytometry: A Novel Platform for Automated High Throughput Drug Discovery and Characterization. J. Biomol. Screen. 6: 83-90, 2001.

     

  3. Kuckuck, F. W., B. S. Edwards and L. A. Sklar: High Throughput Flow Cytometry. Cytometry 44: 83-90, 2001.

     

  4. Jackson, W. C., T. A. Bennett, B. S. Edwards, E. R. Prossnitz, G. P. Lopez and L. A. Sklar: Performance of In-Line Microfluidic Mixers in Laminar Flow for High Throughput Flow Cytometry. Biotechniques 32: 220-226, 2002.

     

  5. Jackson, W. C., F. Kuckuck, B. S. Edwards, A. Mammoli, C. M. Gallegos, G. P. Lopez, T. Buranda and L. A. Sklar: Mixing Small Volumes for Continuous High Throughput Flow Cytometry: Performance of a Mixing Y and Peristaltic Sample Delivery. Cytometry 47: 183-191, 2002.

     

  6. Ramirez, S., C. T. Aiken, B. Andrzejewski, L. A. Sklar and B. S. Edwards: High Throughput Flow Cytometry: Validation in Micro-volume Bioassays.Cytometry A 53A: 55-65, 2003.

     

  7. Chigaev, A., G. Zwartz, S. W. Graves, D. C. Dwyer, H. Tsuji, T. D. Foutz, B. S. Edwards, E. R. Prossnitz, R. S. Larson and L. A. Sklar: a4b1-Integrin Affinity Changes Govern Cell Adhesion. J. Biol. Chem. 278: 38174-38182, 2003.

     

  8. Sklar, L. A., H. Tsuji, B. S. Edwards, R. S. Larson and M. Schuyler: Eosinophil Traffic in the Circulation Following Allergen Challenge. Allergy 59: 596-605, 2004.

     

  9. Young, S. M., M. S. Curry, J. T. Ransom, J. A. Ballesteros, E. R. Prossnitz, L. A. Sklar and B. S. Edwards: High-Throughput Microfluidic Mixing and Multiparametric Cell Sorting for Bioactive Compound Screening. J. Biomol. Screen. 9: 103-11, 2004.

     

  10. Bartsch, J. W., H. D. Tran, A. Waller, A. Mammoli, T. Buranda, L. A. Sklar and B. S. Edwards: An Investigation of Liquid Carryover and Sample Residual for a High-Throughput Flow Cytometer Sample Delivery System. Anal. Chem. 76: 3810-3817, 2004.

     

  11. Chigaev, A., G. J. Swartz, T. Buranda, B. S. Edwards, E. R. Prossnitz and L. A. Sklar: Conformational Regulation of the a4b1-Integrin Affinity by Reducing Agents: “Inside-Out” Signaling is Independent of and Additive to Reduction-Regulated Integrin Activation. J. Biol. Chem. 279: 32435-32443, 2004.

     

  12. Zwartz, G. J., A. Chigaev, D. Dwyer, T. D. Foutz, B. S. Edwards and L. A. Sklar: Real-Time Analysis of VLA-4 Affinity Modulation by Shear. J. Biol. Chem. 279: 32435-32443, 2004.

     

  13. Young, S. M., C. Bologa, E. R. Prossnitz, T. I. Oprea, L. A. Sklar and B. S. Edwards: High Throughput Screening with HyperCyt Flow Cytometry to Detect Small Molecule Formylpeptide Receptor Ligands. J. Biomol. Screen. 10: 374-382, 2005.

     

  14. Oprea, T. I., C. Bologa, B. S. Edwards, E. R. Prossnitz and L. A. Sklar: Post-HTS Analysis: An Empirical Prioritization Scheme. J. Biomol. Screen. 10: 419-426, 2005.

     

  15. Edwards, B. S., C. Bologa, S. M. Young, K. V. Balakin, E. Prossnitz, N. P. Savchuck, L. A. Sklar and T. I. Oprea: Integration of Virtual Screening with High Throughput Flow Cytometry to Identify Novel Small Molecule Formylpeptide Receptor Antagonists. Mol. Pharmacol. 68: 1301-1310, 2005.

     

  16. Bologa, C. G., C. M. Revankar, S. M. Young, B. S. Edwards, J. B. Arterburn, S. E. Tkachenko, N. P. Savchuck, L. A. Sklar, T. I. Oprea and E. R. Prossnitz: Virtual and Biomolecular Screening Converge on a Selective Agonist for GPR30. Nature Chem. Biol. 2: 207-212, 2006.

     

  17. Saunders, M. J., H. Kim, T. A. Woods, J. P. Nolan, L. A. Sklar, B. S. Edwards and S. W. Graves: Microsphere-Based Protease Assays and Screening Application for Lethal Factor and Factor Xa. Cytometry A 69A: 342-352, 2006.

     

  18. Edwards, B. S., S. M. Young, T. I. Oprea, C. Bologa, E. Prossnitz and L. A. Sklar: Biomolecular Screening of Formylpeptide Receptor Ligands with a Sensitive, Quantitative, High-Throughput Flow Cytometry Platform. Nature Protocols 1: 59-66, 2006.

     

  19. Edwards, B. S., S. M. Young, M. J. Saunders, C. Bologa, T. I. Oprea, R. D. Ye, E. R. Prossnitz, S. W. Graves and L. A. Sklar: High Throughput Flow Cytometry for Drug Discovery. Expert Opin. Drug Discovery 2: 685-696, 2007.
Home    Animal Respect Code    Site Map    Employee Links    Contact
© 2008 - 2012 Lovelace Respiratory Research Institute